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杭白菊的快速繁殖及茎尖脱毒培养

时间:2020-01-19 14:56来源:毕业论文
选取杭白菊(Chrysanthemum morifolium Ramat)的嫩芽为外植体,接种在诱导芽分化培养基(MS+0.5mg/L 6-BA)中进行培养,23d左右,植株生长旺盛,再将外植体接种于愈伤组织诱导培养基

摘要选取杭白菊(Chrysanthemum morifolium Ramat)的嫩芽为外植体,接种在诱导芽分化培养基(MS+0.5mg/L 6-BA)中进行培养,23d左右,植株生长旺盛,再将外植体接种于愈伤组织诱导培养基(MS+0.2mg/L NAA +2mg/L 6-BA)中进行继代培养,最后将部分芽接种到生根培养基(1/2MS+0.2mg/L NAA)中培养,15d生根,平均长根数6.63,平均根长3.96cm,21d后进行移栽,移栽后存活率为87.5%。另一部分继代培养的外植体,进行茎尖脱毒,然后在茎尖培养基(MS+0.2mg/L 6-BA)中进行培养,一周后,茎尖开始变绿;13d后,开始出现愈伤组织;28d后愈伤组织变大,有些开始出芽,三个月后菊花茎尖出现褐化,但没有采取措施导致死亡。实验发现, 培养基MS+0.1mg/L NAA +2mg/L 6-BA比较适合丛生芽增殖,平均增殖倍数可达4.9倍;培养基MS+1 mg/L NAA +0.1mg/L 6-BA比较有利于杭白菊根的诱导,每瓶平均长根数5.35根;培养基MS+0.1mg/L NAA +1mg/L 6-BA最适合杭白菊的愈伤组织分化以及芽诱导,芽诱导率有50%。44214

Abstract  Select Chrysanthemum shoots as explants were inoculated in inducing bud differentiation medium (MS + 0.5mg / L 6-BA) were cultured , about 23d, vigorous plant growth , then explants inoculated on callus induction medium (MS + 0.2mg / L NAA + 2mg / L 6-BA) conducted subculture , the last part of shoots inoculated into rooting medium (1 / 2MS + 0.2mg / L NAA) cultured , 15d root, root number average length of 6.63 , the average root length 3.96cm, 21d after transplanting , the survival rate was 87.5% after transplanting . Following another part of explants cultured conduct Virus Free , then shoot tips cultured in medium (MS + 0.2mg / L 6-BA) , and after one week , beginning to turn green shoot tips ; 13d after start appear callus ; 28d after callus larger , some start sprouting , chrysanthemum shoot tip browning after three months , but did not take steps to cause death. It was found that the medium MS + 0.1mg / L NAA + 2mg / L 6-BA is suitable for buds proliferation , proliferation rate of up to 4.9 times the average ; medium MS + 1 mg / L NAA + 0.1mg / L 6-BA Chrysanthemum more conducive to induce root , root number per bottle average length 5.35 ; medium MS + 0.1mg / L NAA + 1mg / L 6-BA callus differentiation as well as the most suitable Chrysanthemum buds , bud induction rate 50 %.

毕业论文关键词:杭白菊;快速繁殖;茎尖脱毒

Keyword: Chrysanthemum morifolium Ramat; rapid propagation ; Virus Free 

目    录

1  引言 5

1.1 杭白菊的介绍及现状 5

1.2 快速繁殖和茎尖脱毒的特点 5

1.3 快速繁殖技术技术介绍 6

1.4 实验研究方法 7

2 实验材料与方法 7

2.1 研究材料 7

2.1.1 植株与材料 7

2.1.2 培养基的配置 7

2.2 主要实验仪器和试剂 8

2.3 主要实验方法 9

2.3.1 材料消毒 9

2.3.2 接种 9

2.3.3 继代培养 9

2.3.4 茎尖脱毒 9

3 结果与分析 10

3.1 初代培养 10

3.2 继代培养 10

3.3 不同浓度激素对杭白菊根诱导的影响 10

3.4 不同浓度激素对丛生芽增殖的影响 杭白菊的快速繁殖及茎尖脱毒培养:http://www.lwfree.com/shengwu/lunwen_45432.html

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